Paxgene rna collection instructions

 

 

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Consult QIAGEN's entire PAXgene Blood miRNA Kit Brochure catalogue on MedicalExpo. Benefits of the PAXgene Blood miRNA System include: I Integrated system for collection, stabilization, and purification RNA stabilization for up to 3 days at 18-25°C or at least 50 months at -20°C or -70°C DATA SHEET. RNA Extraction from RNA-Stabilizing Blood Collection Tubes. It enables purification of high quality RNA with demonstrated compatibility up to 400 µL of blood collected in PAXgene RNA tubes. Oragene•RNA | RE-100. Collection precautions. Do NOT eat, drink, smoke or chew gum for 30 minutes before giving your saliva sample. Discard or recycle outer packaging. Additional notes. Intended use: For the collection of human RNA from saliva samples. The PAXgene Blood RNA System is the first IVD marketed product for the collection, storage, and transportation of blood and stabilization of intracellular RNA in a closed tube with subsequent isolation and purification of intracellular RNA from whole blood for RT-PCR used in molecular diagnostic testing. Using the PAXgene Blood RNA Kit Troubleshooting Guide Appendix A: General Remarks on Handling RNA RNeasy MinElute™ Spin Columns in collection tubes Collection tubes (1.5 ml) Collection tubes (2 Take proper care and check the supplier's instructions. RNeasy Micro Handbook 04/2003. The RNA yields obtained from the blood samples collected in Tempus tubes were consistently higher than from PAXgene tubes. Conclusions: Our results strongly suggest that special collection tubes are necessary for RNA stabilization and they should be used for establishing new biobanks. A protocol for isolation of RNA from whole blood in PAXgene® tubes using the Maxwell® 16 LEV simplyRNA Introduction This Application Note describes a method for purification of total RNA from whole blood 14. Load the rack into the Maxwell® Instrument and follow the on-screen instructions. Peripheral blood is collected in either EDTA or PAXgene tubes. Each collection tube type requires a specified RNA extraction method and RNA input amount. The detailed extraction protocol and RNA quantity requirements are provided in the device package insert and instructions for use. PAXgene Blood RNA Tube. Known as: PAXgene RNA Vacutainer. A blood collection tube that uses proprietary reagents for whole blood collection and stabilization prior to isolation of cellular RNA. Materials Supplied in TruGraf® Collection Kit: • TruGraf® Collection Instructions • TruGraf® Collection Kit (includes kit box, 2 PAXgene. Blood RNA Tubes, sorbent tube wrap, biohazard specimen bag, protective bubblewrap envelope, specimen tube labels. FedEx Clinical/Diagnostic Pak Data are obtained (A) from series A with 500 ng RNA (n = 12; both sampling systems), (B) from series B with 250 ng RNA with additional DNase1 digestion and (C) 5 donors with 4 samples each from TempusTM and PAXgeneTM collection (100 ng each measured in experimental duplicates).

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